透明質(zhì)酸酶,英文名稱Hyaluronidase,一類能夠降低體內(nèi)透明質(zhì)酸活性的酶的總稱,結(jié)構(gòu)上由4個(gè)相同亞基構(gòu)成,每個(gè)亞基分子量接近14kDa,該酶是一種糖蛋白,包含5%的甘露糖和2.2%的葡萄糖胺。功能上該酶可隨機(jī)裂解透明質(zhì)酸、軟骨素和硫酸軟骨素中的β-N-乙酰己糖胺-[1→4]糖苷鍵。常與膠原酶聯(lián)合使用于降解細(xì)胞外基質(zhì),從而從組織中分離活細(xì)胞。本品來(lái)源于牛睪丸,活力值為400–1,000units/mg,合適的pH值為4.5–6.0。產(chǎn)品性質(zhì)中文別名(ChineseSynonym)玻璃糖醛酸酶;玻璃酸酶英文別名(EnglishSynonym)Hyaluronoglucosaminidase;Hyaluronate4-glycanohydrolaseCAS號(hào)(CASNO.)37326-33-3來(lái)源(Source)牛睪丸分子量(MolecularWeight)~55kDa類型(Type)I-S外觀(Appearance)淡黃色至米黃色至褐色粉末重組腸激酶為采用重組大腸桿菌分泌表達(dá)的高純度、高活性、高特異的牛腸激酶,不含其他蛋白酶。Recombinant Mouse SLAMF1/SLAM/CD150 Protein,His Tag
Trop-2,alsoknownasepithelialglycoprotein-1antigen(EGP-1),isaproteinthatinhumansisencodedbytheTACSTD2gene.Mutationsofthisgeneresultingelatinousdrop-likecornealdystrophy,anautosomalrecessivedisordercharacterizedbyseverecornealamyloidosisleadingtoblindness.產(chǎn)品性質(zhì)別名EGP1;EGP-1;TROP2;GA733-1;gp50;T16;TACSTD2;TROP-2;M1S1;TACD2UniprotNo.P09758表達(dá)區(qū)間及表達(dá)系統(tǒng)RecombinantBiotinylatedHumanTROP-2/TACSTD2ProteinisexpressedfromHEK293cellswithHistagandAvitagattheC-terminal.ItcontainsHis27-Thr274.分子量TheproteinhasapredictedMWof30.5kDa.Duetoglycosylation,theproteinmigratesto46-55kDabasedonTris-BisPAGEresult.純度>95%asdeterminedbySDS-PAGEandHPLC活性ELISAData:ImmobilizedAnti-TROP-2Antibody,hFcTagat0.5μg/mL(100μL/well)ontheplate.DoseresponsecurveforBiotinylatedHumanTROP-2,HisTagwiththeEC50of24.1ng/mLdeterminedbyELISA.0EUper1μgoftheproteinbytheLALmethod.制劑Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally5%trehaloseisaddedasprotectantbeforelyophilization.Recombinant Human Her4/ErbB4 Protein,hFc TagCX3CL1還通過(guò)募集巨噬細(xì)胞以及通過(guò)募集和介導(dǎo)破骨細(xì)胞前體的粘附而導(dǎo)致傷口愈合。
3C蛋白酶是切割小RNA病毒科非結(jié)構(gòu)蛋白的關(guān)鍵酶,在病毒復(fù)制過(guò)程中發(fā)揮著重要作用。鼻病毒屬于小RNA病毒科,其中的人鼻病毒3C蛋白酶基因編碼區(qū)全長(zhǎng)552bp,編碼的蛋白質(zhì)相對(duì)分子質(zhì)量約為22000Da。人鼻病毒3C蛋白酶具有高度的酶切特異性,能特異切割位于Gln-Gly之間的肽鍵,識(shí)別位點(diǎn)為L(zhǎng)eu-Glu-Val-Leu-Phe-Gln↓Gly-Pro。本公司將人鼻病毒3C蛋白酶的編碼區(qū)基因,在大腸桿菌中進(jìn)行重組表達(dá),純化后獲得了高純度的重組3C蛋白酶,該蛋白酶能特異切割含有3C酶切位點(diǎn)的融合蛋白,具有良好的生物學(xué)活性。同時(shí),本公司生產(chǎn)的3C蛋白酶帶有GST和MAT標(biāo)簽蛋白,有利于后期將其從酶切體系中去除。產(chǎn)品性質(zhì):中文別名(Chinesesynonym):3C蛋白酶英文別名(Englishsynonym):3CProtease來(lái)源(Source):大腸桿菌表達(dá)標(biāo)簽(label):GST-3CProtease-MAT純度(Purity):經(jīng)SDS-PAGE及HPLC分析,純度>95%分子量(Molecularweight):47.38kDa比活性(Specificactivity):500U/ml緩沖液組分(Buffer):50mMTris-HCl,150mMNaCl,1mMEDTA,1mMDTT,pH7.0酶活定義(UnitDefinition):在緩沖液中切割100μg被檢測(cè)的融合蛋白,反應(yīng)條件為4℃16小時(shí),切割率≥90%運(yùn)輸和保存方法:干冰運(yùn)輸;保存于-20℃。
另外,也提供來(lái)源于牛肺的抑肽酶(動(dòng)物源性)。產(chǎn)品信息規(guī)格1mg/10mg產(chǎn)品性質(zhì)來(lái)源(Source)大腸桿菌表達(dá)CAS號(hào)(CASNO.)9087-70-1外觀(Appearance)白色、類白色、類黃色粉末溶解度(5mg/mlin0.9%NaCl)可溶,澄清或微濁純度(Purity)≥95%(HPLC)蛋白含量(Proteincontent)≥60%酶濃度(EnzymeConcentration)≥3.0EPU/mgpro活性定義(ActivityDefinition)胰蛋白酶單位:每秒鐘能水解1μmoL的N-苯甲酰-L-精氨酸乙酯(BAEE)為1個(gè)胰蛋白酶單位。酶活單位:能抑制1個(gè)胰蛋白酶單位的活力稱為1個(gè)抑肽酶活力單位(EPU)。儲(chǔ)存條件凍干粉2~8℃保存,有效期2年。使用方法抑肽酶與蛋白酶是等摩爾有效結(jié)合,推薦的結(jié)合pH>6.0,在pH<3.0的條件下不結(jié)合??芍苯邮褂?.9%NaCl溶解,溶解后可-20℃儲(chǔ)存。eotaxin-2還具有抑制髓樣細(xì)胞增殖的能力,髓樣細(xì)胞增殖是Eotaxin不共有的生物學(xué)功能。
重組型TEV蛋白酶(rTEV)是經(jīng)過(guò)基因工程改造和純化后的重組蛋白酶,不僅保持天然TEV酶的功能活性,且在廣譜的溫度范圍內(nèi)表現(xiàn)出更強(qiáng)的穩(wěn)定性和特異性。rTEV是一種用來(lái)切除融合蛋白上親和標(biāo)簽的常用工具酶,具有很強(qiáng)的位點(diǎn)特異性,嚴(yán)格識(shí)別七氨基酸序列EXXYXQ↓(G/S),切割位點(diǎn)在谷氨酰胺和甘氨酸/絲氨酸之間。普遍應(yīng)用的七氨基酸序列為:Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly。rTEV在pH7.0,30℃時(shí)可達(dá)活性,但在pH6.0-8.5和溫度4-30℃范圍內(nèi)皆有活性(見(jiàn)表1),使得反應(yīng)條件的選擇可根據(jù)目的蛋白的情況而靈活變動(dòng)。另外rTEV切割后也能利用其N(xiāo)端的6×His標(biāo)簽,通過(guò)Ni-NTA樹(shù)脂去除,以達(dá)到純化目的蛋白的目的。產(chǎn)品性質(zhì)來(lái)源(Source)大腸桿菌外觀(Appearance)無(wú)色透明液體比活力(SpecificActivity)5U/μL活性定義(UnitDefinition)在1×rTEVBuffer(50mMTris,pH8.0,0.1mMEDTA,1mMDTT)中,30℃反應(yīng)1h,剪切>85%的3μg底物所需要的酶量定義為一個(gè)活性單位。純化(Purification)Ni柱親和純化純度(Purity)≥95%(bySDS-PAGE)產(chǎn)品組分運(yùn)輸與保存方法冰袋運(yùn)輸。透明質(zhì)酸酶,英文名稱Hyaluronidase,一類能夠降低體內(nèi)透明質(zhì)酸活性的酶的總稱,結(jié)構(gòu)上由4個(gè)相同亞基構(gòu)成。Recombinant Human Complement Factor D/CFD Protein,hFc Tag
重組腸激酶(rEK)是一種高純度的重組牛腸激酶輕鏈片段,氨基酸序列與牛腸激酶輕鏈一致,有著酶切位點(diǎn)。Recombinant Mouse SLAMF1/SLAM/CD150 Protein,His Tag
SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS?CoV and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 .The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Before binding to the ACE-2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy. Polyclonal antibodies to the RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy. Recombinant Mouse SLAMF1/SLAM/CD150 Protein,His Tag
在分子生物學(xué)實(shí)驗(yàn)中,PCR技術(shù)是基因擴(kuò)增的重要工具,而Pfu Master Mix (2×) (With Dye) 則是結(jié)合了高保真性和便捷性的理想選擇。這種預(yù)混液不僅繼承了Pfu DNA聚合酶的重要的保真性,還通過(guò)添加熒光染料,為實(shí)驗(yàn)提供了更直觀的監(jiān)測(cè)手段。Pfu Master Mix (2×) (With Dye) 是一種即用型的2倍濃度預(yù)混液,含有Pfu DNA聚合酶、dNTPs、優(yōu)化的反應(yīng)緩沖液以及用于實(shí)時(shí)監(jiān)測(cè)的熒光染料。Pfu DNA聚合酶以其高保真性著稱,其3'-5'外切酶活性能夠在DNA合成過(guò)程中糾正錯(cuò)誤摻入的堿基,提高擴(kuò)增產(chǎn)物的準(zhǔn)確性。與普通Taq酶相比,Pfu酶的錯(cuò)誤率更低,...