CD30活性艾利莎數(shù)據(jù):固定化抗CD30抗體,板上的氫氟碳化合物標(biāo)記為0.5歐氏/毫升(100歐氏/井)。生物化人CD30的劑量反應(yīng)曲線,以49ng/ml的EC50作為標(biāo)記。艾麗莎數(shù)據(jù):固定了人的CD30配體,他的標(biāo)簽在板上5g/ml(100ml/井)。用酶聯(lián)免疫吸附法測(cè)定人CD30的劑量反應(yīng)曲線,標(biāo)記為27.1ng/ml的EC50。產(chǎn)品用Lal法測(cè)定每一種蛋白質(zhì)。制劑在PBS(PPS)中用0.22離子過(guò)濾溶液凍干(PH7.4)。通常在凍干前添加5%海藻糖作為保護(hù)劑。復(fù)溶離心管打開(kāi)前。建議重組到超過(guò)100克/毫升的濃度(凍干通常使用1毫克/毫升溶液)。在蒸餾水中溶解凍干蛋白。運(yùn)輸與保存方法冰袋運(yùn)輸。-20℃至-80℃保存,一年有效期。復(fù)溶后,-20至-80°C,在未開(kāi)封狀態(tài)下保存3-6個(gè)月。復(fù)溶后,2-8°C保存2-7天。建議使用時(shí)分裝凍存,避免反復(fù)凍融。注意事項(xiàng)1.避免反復(fù)凍融。2.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。3.本產(chǎn)品作科研用途!產(chǎn)品數(shù)據(jù)成纖維細(xì)胞生長(zhǎng)因子18(FGF-18)是20 kDa蛋白,在骨骼發(fā)育和骨穩(wěn)態(tài)中起著重要作用。Recombinant Mouse APLN Protein,hFc Tag
SARS-CoV-2,whichcausestheglobalpandemiccoronavirusdisease2019(Covid-19),belongstoafamilyofvirusesknownascoronaviruses.TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).SeveralemergingSARS-CoV-2genomeshavebeenidentifiedincludingtheOmicron,orB.1.1.529,variant.FirstidentifiedinNovember2021inSouthAfrica,theOmicronvariantquicklybecamethepredominantSARS-CoV-2variantandisconsideredavariantofconcern(VOC).TheOmicronvariantcontains15mutationsinRBDdomainthatpotentiallyaffectviralfitnessandtransmissibility.ThemajorityofthemutationsareinvolvedinACE-2bindingandOmicronbindsACE-2withgreateraffinity,potentiallyexplainingitsincreasedtransmissibility.Severalofthesemutationsarealsoidentifiedinfacilitatingimmuneescapeandreducingneutralizationactivitytoseveralmonoclonalantibodies.Recombinant Human ITGB6 Protein,His Tag內(nèi)分泌腺衍生的血管內(nèi)皮生長(zhǎng)因子(EG-VEGF),也稱為(PK1),是分泌蛋白的原蛋白蛋白家族的成員。
透明質(zhì)酸酶,英文名稱Hyaluronidase,一類能夠降低體內(nèi)透明質(zhì)酸活性的酶的總稱,結(jié)構(gòu)上由4個(gè)相同亞基構(gòu)成,每個(gè)亞基分子量接近14kDa,該酶是一種糖蛋白,包含5%的甘露糖和2.2%的葡萄糖胺。功能上該酶可隨機(jī)裂解透明質(zhì)酸、軟骨素和硫酸軟骨素中的β-N-乙酰己糖胺-[1→4]糖苷鍵。常與膠原酶聯(lián)合使用于降解細(xì)胞外基質(zhì),從而從組織中分離活細(xì)胞。本品來(lái)源于牛睪丸,活力值為400–1,000units/mg,合適的pH值為4.5–6.0。產(chǎn)品性質(zhì)中文別名(ChineseSynonym)玻璃糖醛酸酶;玻璃酸酶英文別名(EnglishSynonym)Hyaluronoglucosaminidase;Hyaluronate4-glycanohydrolaseCAS號(hào)(CASNO.)37326-33-3來(lái)源(Source)牛睪丸分子量(MolecularWeight)~55kDa類型(Type)I-S外觀(Appearance)淡黃色至米黃色至褐色粉末
Recombinant Biotinylated Human MSLN/Mesothelin Protein,hFc-Avi Tag分子別名(Synonyms)Mesothelin;CAK1;MSLN;MPFSMRP表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanMSLN/MesothelinProteinisexpressedfromHEK293withhFctagandAvitagattheC-Terminus.ItcontainsGlu296-Gly580.[Accession|Q13421-2]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof61.1kDa.Duetoglycosylation,theproteinmigratesto70-80kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.活性(Activity)ELISAData:ImmobilizedAnti-MSLNAntibody,hFcTagat1μg/ml(100μl/well)ontheplate.DoseresponsecurveforBiotinylatedHumanMSLN,hFcTagwiththeEC50of18.4ng/mldeterminedbyELISA.LAG-1與MIP-1β(ACT II同種型)相同,Lag-1趨化吸引力單核細(xì)胞,并表現(xiàn)出活性作為HIV抑制因子。
SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS?CoV and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 .The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Before binding to the ACE-2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy. Polyclonal antibodies to the RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy. 泛素化反應(yīng)可以修飾蛋白質(zhì),調(diào)節(jié)蛋白質(zhì)降解。泛素化還影響蛋白質(zhì)體事件,如蛋白質(zhì)定位、活性和功能。Recombinant Mouse CD52 Protein,hFc Tag
IdeS 具有獨(dú)特的底物選擇性,可以作為工具酶應(yīng)用于抗體類藥物或抗體融合蛋白藥物的結(jié)構(gòu)表征分析。Recombinant Mouse APLN Protein,hFc Tag
糖苷酶F(PNGaseF)是一種酰胺水解酶,經(jīng)過(guò)和平空間站伊麗莎菌克隆,主要由腦膜炎膿桿菌等革蘭氏陰性菌分泌。N-糖苷酶F(PNGaseF)在酵母中重組表達(dá)(比活性:100000U/mL),可以裂解由天冬酰胺連接的高甘露糖、雜合和復(fù)雜的寡糖糖蛋白。PNGaseF的切割位點(diǎn)為糖蛋白內(nèi)側(cè)N-乙酰葡萄糖胺(GlcNAc)和天冬酰氨殘基之間的酰胺鍵,同時(shí)將酶解后蛋白上的天冬氨酰轉(zhuǎn)化為天冬氨酸。本產(chǎn)品帶his標(biāo)簽,常應(yīng)用于抗體及其相關(guān)蛋白完全去糖基化。另外,我司還提供其他類型的糖苷酶,包括酵母重組表達(dá)的N-糖苷酶F(比活性:750000U/mL),內(nèi)切糖苷酶H,內(nèi)切糖苷酶S。產(chǎn)品信息產(chǎn)品性質(zhì)中文別名(Chinesesynonym)N-糖酰胺酶F;N-糖苷酶F英文別名(Englishsynonym)PNGaseF來(lái)源(Source)酵母重組表達(dá)分子量(Molecularweight)36kDa比活性(Specificactivity)100000U/mL緩沖液組分(Buffer)20mMTris-HClpH7.5,50mMNaCl,5mMEDTA,50%GlycerolRecombinant Mouse APLN Protein,hFc Tag
在分子生物學(xué)實(shí)驗(yàn)中,PCR技術(shù)是基因擴(kuò)增的重要工具,而Pfu Master Mix (2×) (With Dye) 則是結(jié)合了高保真性和便捷性的理想選擇。這種預(yù)混液不僅繼承了Pfu DNA聚合酶的重要的保真性,還通過(guò)添加熒光染料,為實(shí)驗(yàn)提供了更直觀的監(jiān)測(cè)手段。Pfu Master Mix (2×) (With Dye) 是一種即用型的2倍濃度預(yù)混液,含有Pfu DNA聚合酶、dNTPs、優(yōu)化的反應(yīng)緩沖液以及用于實(shí)時(shí)監(jiān)測(cè)的熒光染料。Pfu DNA聚合酶以其高保真性著稱,其3'-5'外切酶活性能夠在DNA合成過(guò)程中糾正錯(cuò)誤摻入的堿基,提高擴(kuò)增產(chǎn)物的準(zhǔn)確性。與普通Taq酶相比,Pfu酶的錯(cuò)誤率更低,...